Composite

Part:BBa_K3046022

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-07-04)


PyrG selection marker from A. fumigatus

This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants, such as the NIG96 Aspergillus niger strain.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
    Illegal PstI site found at 852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 14
    Illegal PstI site found at 852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 14
    Illegal BamHI site found at 39
    Illegal BamHI site found at 590
    Illegal XhoI site found at 450
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
    Illegal PstI site found at 852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 14
    Illegal XbaI site found at 45
    Illegal PstI site found at 852
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None